WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Pattern

LET-653 is expressed by all vulva cell types. The ZP proteins FBN-1 and LET-653 showed somewhat complementary luminal patterns. Beginning at L4.2, and as previously reported for LET-653(PAN domain) fragments (Gill et al., 2016), LET-653 decorated a core structure in the center of the lumen that rises to the level of the vulD and vulE cells, along with lateral elements that connect this core to the vulA, vulB1 and vulB2 cells and to the surrounding epidermis. The central core structure could also be detected very weakly by DIC. This core changed appearance during vulva eversion, but remained visible in the most ventral, 2 ̊-cell-derived region through the L4.9 stage. Transiently, at the L4.3-L4.5 stages, LET-653 also weakly marked the apical membranes of most cells. Finally, FBN-1 overlapped with LET-653-marked structures near vulB1 and vulB2 surfaces, but otherwise was mainly excluded from the core area and instead filled the more dorsal part of the lumen above the core. During vulva eversion, FBN-1 became excluded from the dorsal-most portions of the lumen lined by 1 ̊-derived cells, such that LET-653 and FBN-1 together appeared to demarcate at least three separate luminal zones roughly corresponding to the regions outlined by the vulA/B cells, vulC/D cells, and vulE/F cells.

Primary Identifier

Expr15951