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Expr4442
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T23D8.1 is expressed in a few cells in the nerve ring, putatively identified as neurons. In addition, there is also expression in the intestine, in one cell on the ventral side of the terminal pharyngeal bulb and two cells around the anus. |
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Picture: Figure 5a, 5b.. |
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Expr8586
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Transgenic C. elegans expressing the cuti-1::gfp translational transgene (WT030) displayed GFP expression in the developing hypodermis of mid- and late-stage embryos. GFP expression was also observed in the epithelia throughout development until the fourth-stage larva. At no time was GFP observed in adult stages. Expression was seen exclusively in the cytoplasm of the hypodermis, in the vulval and anal epithelium, and in the excretory pore. Strong expression was also observed in the seam cells and at the seam cell boundary. All of these tissues are covered by cuticle or involved in cuticle synthesis and secretion. |
Within the hypodermis, GFP expression was observed at small discrete points, possibly corresponding to vesicle-like structures. |
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Expr13873
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Bright fluorescence was detected in post-gastrulating embryos in regions corresponding to intestinal cells and the dorsal body wall muscle quadrant. Expression continued throughout all larval stages and into adulthood in most tissues except for the gonadal cells. By mid L4, GFP fluorescence was visible in intestinal and muscle cells, with concentrated regions within the pharyngeal and vulval muscles. There was an obvious change in the expression pattern of young adults as fluorescence decreased in the anterior head and posterior tail regions, but the intestine, pharynx and particularly the spermatheca continued to exhibit bright reporter expression. Fluorescence was equally observed in the excretory system within structures resembling the excretory gland cells, the intestinal-rectal valve and anus. Interestingly, no expression was observed in the male gonad. |
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Expr9183
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UEV-1 is broadly expressed in the pharynx, neurons, muscle cells, vulva, embryos, intestine, and in the anus/tail. We found strong expression in the distal tip cell. We also found expression in the motoneurons that line the ventral cord. |
We noted that the UEV-1::GFP chimeric protein is localized diffusely throughout the cytosol of most cells, but with some enrichment in the nucleus. |
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Picture: Figure 2, Fig. S2BE. |
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Expr8581
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The expression of genomic magi-1::gfp and magi-1::rfp chimeric transgenes, which contain 4 kb of upstream sequence and either the complete magi-1 ORF or only the genomic magi-1 long isoform sequences, yielded similar results The magi-1L:::gfp reporter transgene reflects the expression of the long isoform. Expression of MAGI-1L::GFP was detected in the pharynx, head neurons, ventral cord, and vulva of animals mosaic for the extrachromosomal array. Expression of MAGI-1L::RFP was also detected in the pharynx, intestine, vulval epithelia (L4 stage), and vulval muscle (adult stage) of animals mosaic for the extrachromosomal array. This antibody labeled both neurons and epithelial junctions in immunofluorescence experiments. Within the hypodermis and intestine, there was a high degree of colocalization between MAGI-1 and DLG-1. The anus and vulva also were labeled in larval and adult animals. Finally, MAGI-1 expression was observed in some neurons in the head, including a subset of the GLR-1::GFP-expressing command interneurons. MAGI-1 is expressed in the backward but not the forward command interneurons; MAGI-1 expression was not detected in any of the mechanosensory neurons. MAGI-1 was detected in the neurons AVA, AVD, and SMD, as well as RMD and RMDV. |
Anti-MAGI-1 antibody detected MAGI-1 localization to puncta along the ventral cord; however, MAGI-1 is expressed in more neurons than just those that express GLR-1, making analysis of colocalization in the ventral cord difficult. To help restrict the analysis to the GLR-1-expressing neurons, both MAGI-1 isoforms were expressed as mRFP chimeric proteins under the glr-1 promoter; both substantially colocalized with GLR-1. |
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Picture: Figure 3. |
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Expr8736
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pF55H12.4::GFP expressed in hypodermis, vulva, anus, and to a lesser extent pharynx. Hypodermal expression was highly variable and was strongest in L4 and Adult stages. |
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Other strains-- UL1009, UL1015, UL1012 |
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Expr2035
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Very strong diffuse expression is seen throughout the pharynx of larval and adult stages. In young larvae expression is also seen in cells along the body of the worm (probably hypodermal seam cells), and in a few cells of the anal region. |
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Expr16580
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We used a putative promoter 5 kb sequence immediately upstream of the start codon of pezo-1 isoforms i and j. GFP expression was mainly restricted to pharyngeal muscles and sensory neurons. In the pharynx, we saw GFP labeling of the pm3 muscle cells, the mc1 marginal cells in the corpus, a structure responsible for generating suction forces during the ingestion of suspended bacteria (Raizen et al. 2012), and in a neuron consistent in location and morphology with I4. A ventral cell located posterior to the terminal bulb and extending a process dorso-anteriorly toward the pharyngeal-intestinal valve (consistent with the excretory gland) was also labeled. Based on their location and characteristic extensive branching, we identified the anterior and posterior mechanoreceptors FLP and PVD (respectively) as also expressing GFP. Posteriorly, we tentatively identified a phasmid neuron consistent with the PHC neuron's morphology and location, as well as other cells associated with the uterus and anus, as expressing GFP. Unlike the other transcriptional constructs, GFP expression in these animals was visible as early as prezle-stage embryos. |
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No GO_term assigned. |
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Expr17
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Expression first becomes evident at the first larval stage, L1, when the head body-wall muscles and an unidentified cell(s) in the anal region harbour stain. The anal staining lasts only until L2/3, whilst the head muscles continue showing expression right through to adulthood. The expression pattern includes a further component in the vm1 cells associated with the vulva. These specialised muscle cells attach to the ventral hypodermis and the vulval labia, and are important in opening the vulva during egg-laying. Expression here is transient, seen only for a short time around the L4 to adult molt. All components of the expression pattern are non-nuclear localised. The nuclei of the vm1 vulval muscle stain up, as do body wall muscles in the head. Vulval staining can be seen L4-young adult, with the head muscles staining earlier al ~L1-L3. |
Expression is non-nuclear localized. |
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Reporter gene fusion type not specified. |
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Expr2312
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Signals were detected in some neurons (ASE, ADF, AVA, AUA, RMDV and BAG) in the head region, the anchor cell, the vulva, the cells around the anus, the body wall muscle, pharyngeal muscles in procorpus and metacorpus and distal tip cells of gonad. |
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Reporter gene fusion type not specified. |
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Expr2313
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Signals were detected in some neurons (ASE, ADF, AVA, AUA, RMDV and BAG) in the head region, the anchor cell, the vulva, the cells around the anus, the body wall muscle, pharyngeal muscles in procorpus and metacorpus and distal tip cells of gonad. |
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Expr13426
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amx-2 was localized in pharyngeal muscle, head neurons including neurosecretory motor neurons, vulva, HSN, intestine, and anus. amx-2 expression has significant overlap with grk-2 especially in the HSN and vulva. |
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Expr9281
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glp-1p::GFP expression is observed near the excretory pore, anus, in vulval precursor cells, intestinal cells, and in a few head neurons that could not be identified due to low expression levels. |
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Reporter gene fusion type not specified. src-2 is called kin-22 in this paper. |
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Expr2314
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Expressed in pharyngeal muscles, the vulva and some cells around anus. |
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