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Since utse aff-1 expression and AC-utse fusion occur almost simultaneously, it is possible that aff-1 expression detected in the utse is actually a contribution from the AC cytoplasm after the fusion event. To test this, authors examined utse aff-1 expression in lin-29(n482) mutant worms where AC-utse fusion does not occur. aff-1 expression in the utse in these mutants indicated that aff-1 is specifically expressed in the utse cells and is not a consequence of AC to utse cytoplasmic GFP diffusion after fusion. |
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Expr4654
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Specific and continuous expression was detected in the AC from the invasion of the vulval primordium at mid-L3 until its fusion with the utse cells. As the vulva completes its invagination in the L4, the utse syncytium starts to express aff-1, resulting in coexpression of aff-1 in both cells prior to their fusion. Thus, AFF-1 is dynamically expressed in a specific group of cells that undergo cell fusion during normal development. aff-1 expression is first detected in the embryonic hyp5 cell and later during larval development in various cell types, including pharyngeal muscles (Pm3 and Pm5), uterine rings (Ut2 and Ut4), head and tail neurons, sheath cells of chemosensory neurons, and male tail neurons). aff-1 is also expressed in vulval vulD and the seam cells shortly before these cells fuse. In general, the myoepithelial cells of the pharynx and the epithelial cells in the uterus, vulva, and hypodermis that express aff-1 undergo fusion. Thus, AFF-1 is dynamically expressed in a specific group of cells that undergo cell fusion during normal development. |
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Fusion junction is at position 21392 in F18G5 ...GTATTCTCTGAGAGAAGGAATGATC/lacZ Young and Hope (1993). Dev. Dynam. 196:124-132 = [cgc1752]. Legacy Data: Author "Arnold JM" "Guo J" "Hope IA"Date 1992-01. life_stage summary : postembryonic |
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Expr51
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b-galactosidase expression in the spermathecae and the three rectal epithelial cells. Staining in the rectal area was first observed in L1 larvae whilst expression in the spermathecae appeared as the structure formed in L4 larvae. Variable staining was also seen throughout the uterus. In the mature gonad staining appeared to be in the two sets of two large toroidal epithelial cell, Ut-1 and Ut-2. Staining was also observed in the large, multinucleate H-shaped Use cell which attaches the uterus to the seam cells and to the four epithelial cells Ut-1 and Ut-2. The Uv-1 cells did not appear to stain. Individual worms often showed only one component of this expression pattern. In the male, expression was observed to be dispersed in what appeared to be all or part of the proctodeum. |
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Expr1164
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Transgenic animals that carry this construct show GFP expression in a variety of tissue types. GFP expression is observed in the intestine, and the posterior cells express GFP more intensely than the remaining intestinal cells. Other cells include the rectal epithelial cells, the pharynx, the somatic gonad, and vulval hypodermal cells. In addition, the IP3 receptor is expressed in hypodermal cells of the tail, rectum, and head. Pharyngeal expression is restricted to the muscles of the metacorpus, isthmus, and the anterior portion of the terminal bulb (m4, m5, and m6). This construct was only expressed in neurons LUA and PDA. GFP is expressed in the gonad sheath cells, spermatheca, spermathecal valve, and uterine sheath cells. GFP is expressed in the vulval hypodermal cells. |
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Expr3206
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In addition to expression in a number of neurons (RMEL/R, RID, BDUL/R, AIYL/R, AVHL/R, AIZL/R, ALNL/R, RICL/R, RIAL/R and PDA) as has been reported previously, intense fluorescence was also observed in uterine toroid cells (ut1 and ut2). |
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Expr1816
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All three reporter constructs were expressed in all major contractile tissues, starting during embryogenesis and continuing until adulthood. Fluorescence was particularly pronounced in striated muscle (body wall muscles used for locomotion), non-striated muscle (pharyngeal muscles used for pharyngeal pumping, vulval and uterine muscles used for egg laying, the sphincter muscle and anal depressor used for defecation), and in myoepithelial cells (gonadal sheath cells used for ovulation). Furthermore, all three constructs showed expression in the intestine. Some cells expressed CeSERCAb::GFP but not CeSERCAa::GFP. These include the somatic cells of the spermatheca and the excretory canal and the uterine sheath cells. No cells were found that expressed CeSERCAa::GFP but not CeSERCAb::GFP. |
Fluorescence from Psca-1::GFP was evenly distributed over the cytosol. However, both CeSERCAa::GFP and CeSERCAb::GFP showed specific subcellular localization. Both fusion proteins were found in a tubular meshwork that was most distinct in the body wall muscle cells. This staining was continuous with staining at or near the dense bodies, structures functionally analogous to vertebrate Z-lines. In body wall muscle cells and other cells, staining was also often localized in internal vesicles and membrane-like structures, including the nuclear envelope. |
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Other strains-- UL870, UL874 |
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Expr426
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Diffuse non-nuclear expression is seen in older larvae and adults in three distinct components, i.e. the excretory cell and canals, the posterior hypodermis, and the uterine wall (ut2 toroidal cells). This gene has similarity to amino acid transporters. |
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[CEH-63::gfp] translational fusion. |
Expr9877
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CEH-63 expression was observed in DVC and the uterus. The GFP signal was localized to the cell nuclei, presumably due to the DNA binding activity of the transcription factor taking the fusion protein to the nucleus. The nuclear localization allowed identification of the uterine cells expressing the CEH-63 C-terminal GFP fusion as ut2 and ut3. This identification was made in young adults after uterine development was complete. Although no extra expression was observed for the N-terminal fusion, for the C-terminal fusion there was also reliable expression in 6-10 nuclei in the head region and at a similar level to that in DVC. There was also weak but frequent expression of the C-terminal fusion in two other unidentified nuclei, posterior to DVC, in the hermaphrodite tail. CEH-63 expression was observed in DVC and the uterus. The GFP signal was localized to the cell nuclei, presumably due to the DNA binding activity of the transcription factor taking the fusion protein to the nucleus. The nuclear localization allowed identification of the uterine cells expressing the CEH-63 C-terminal GFP fusion as ut2 and ut3. This identification was made in young adults after uterine development was complete. Although no extra expression was observed for the N-terminal fusion, for the C-terminal fusion there was also reliable expression in 6-10 nuclei in the head region and at a similar level to that in DVC. There was also weak but frequent expression of the C-terminal fusion in two other unidentified nuclei, posterior to DVC, in the hermaphrodite tail. |
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To cite this work: Ghosh, Srimoyee; Inoue, Takao; Sternberg, Paul (2015): ina-1::gfp expression. WormBase. Dataset. DOI: http://dx.doi.org/10.17912/W2159J . Reviewed by Bhagwati Gupta. Funding: National Institutes of Health USPHS training grant GM07616. |
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Expr12292
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ina-1 is expressed throughout uterine toroids. At mid L4, expression is seen in uterine toroid 1 and 4 (ut1, ut4) and in the anterior uterine toroids 2 and 3 (ut2, ut3). In addition, ina-1::GFP is detected in the spermathecal-uterine junction and spermatheca. At the L4 lethargus stage, bright expression is observed in uterine toroids 1 and 4, the spermathecal-uterine junction, and spermatheca. Dimmer expression is present in uterine toroid 2 and posterior toroid 3. Fluorescence is also detected in vulE. |
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