WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Expression Pattern :

Primary Identifier  Expr4322 Subcellular Localization  MEL-28 was expressed in embryos as well as in all larval stages and in adults. Immunolocalization of MEL-28 in several tissues revealed a nuclear-rim localization typical for NE or NPC proteins. MEL-28 was localized to chromatin during mitosis. The localization of MEL-28 within the NE was then analyzed by immuno-gold TEM. As expected, the highest density of gold particles was observed at the NE, but particles were also observed in the nucleoplasm and the cytoplasm. Particles found in the cytoplasm most likely reflect a cross reactivity of the MEL-28 antibody because a similar density of gold particles was observed in the cytoplasm of mel-28(t1684) embryos. No gold particles were observed in the nuclei or at the nuclear periphery of mutant embryos. Of the particles associated with the NE in the wild-types, 95.0% (152/160) were found at NPCs. No accumulation at the outer nuclear membrane was detected. MEL-28 enrichment at NPCs was also detected in gonad nuclei. Immunofluorescence analysis revealed that MEL-28 began to localize to the condensing chromosomes before complete disappearance of mAb414 staining from the nuclear rim at prophase and began to appear as two lines parallel to the metaphase plate. During late anaphase, MEL-28 signal colocalized with the decondensing chromosomes, and the mAb414 signal reaccumulated to the reforming NE during telophase. Immunolocalization of MEL-28 in embryos expressing GFP-HIM-10 revealed an overlapping localization of both proteins parallel to the metaphase plate within the mitotic spindle. Only a few kinetochore microtubules remained visible after 30 s on ice, and after 60 s, microtubules were not detectable. At both time points, MEL-28 staining remained clearly visible along the condensed and aligned chromosomes, indicating that MEL-28 localizes to kinetochores during mitosis. MEL-28 localization changes rapidly at late anaphase and telophase. MEL-28 initially spreads over the entire chromatin surface then rapidly relocalizes to the NE. This distribution would be consistent with MEL-28's playing a role in the interactions that occur between chromatin and the assembling NE and NPCs late in mitosis.
Paste the following link
Lists
This ExpressionPattern isn't in any lists. Upload a list.

0 Anatomy Terms

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00003210 mel-28 C38D4.3 Caenorhabditis elegans

6 Life Stages

Remark Definition Other Name Public Name Primary Identifier
  The stage that begins when a C.elegans individual is fully-developed and has reached maturity. adult Ce WBls:0000041
  The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch. L3 larva Ce WBls:0000035
  The second stage larva. At 25 Centigrade, it ranges 25.5-32.5 hours after fertilization, 11.5-18.5 hours after hatch. L2 larva Ce WBls:0000027
  The fourth stage larva. At 25 Centigrade, it ranges 40-49.5 hours after fertilization, 26-35.5 hours after hatch. L4 larva Ce WBls:0000038
  The first stage larva. At 25 Centigrade, it ranges 14-25.5 hours after fertilization, 0-11.5 hours after hatch. L1 larva Ce WBls:0000024
  The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching. embryo Ce WBls:0000003