1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000553 | cmk-1 | K07A9.2 | Caenorhabditis elegans |
| WormBase ID | WBStrain00062969 | CGC Received | 2024-08-20 |
| Genotype | cmk-1(sy2277[cmk-1::mKate2::AID*::3xFLAG) IV; syIs875. | Laboratory | CGC |
| Mutagen | CRISPR_Cas9 | Name | PS10640 |
| Outcrossed | x0 | Remark | syIs875 [ins-6p::dYFP + ins-6::mCherry + unc-122p::GFP]. cmk-1(sy2277) is a C-terminal knock-in of mKate2::AID::FLAG to be used for conditional degradation of CMK-1 protein. sy2277 is a CRISPR-engineered allele generated using the self-excising cassette (SEC) method (Dickinson et al. 2015, Genetics) with the gRNA sequence 5'-AGCGTGAAAAGCGGGTGTAGNGG-3' (note: NGG not included in the gRNA). syIs875 is an integrated transgene that includes a transcriptional and translational reporter for ins-6 and is marked by GFP in the coelomocytes. dYFP signal can be seen in ASI during reproductive growth and in ASJ (strong) and ASI (weaker) during dauer exit. Reference: Zhang MG, et al. (2024). Available at: https: |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000553 | cmk-1 | K07A9.2 | Caenorhabditis elegans |
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