3 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001067 | dpy-5 | F27C1.8 | Caenorhabditis elegans |
| WBGene00004479 | rps-10 | D1007.6 | Caenorhabditis elegans |
| WBGene00006753 | unc-14 | K10D3.2 | Caenorhabditis elegans |
| WormBase ID | WBStrain00062917 | CGC Received | 2024-02-26 |
| Genotype | rps-10(cc2557)/tmC20 [unc-14(tmIs1219) dpy-5(tm9715)] I. | Laboratory | CGC |
| Mutagen | CRISPR_Cas9 | Name | PD2557 |
| Outcrossed | x2 | Remark | Balancer recombination happens frequently at 23-25C, strain must be maintained at 16-20C. Homozygous lethal mutation balanced by Dpy- and myo-2p::Venus-marked inversion. Heterozygotes are non-Dpy with relatively dim pharyngeal GFP (Venus) expression, and segregate heterozygous non-Dpy Venus+, non-Venus cc2557 homozygotes (L1 arrest), and Dpy with brighter Venus+ (tmC20 homozygotes). Pick wild-type Venus(+) and check for proper segregation of progeny to maintain. cc2557 is an engineered mutation creating an early stop (T8*). Presumptive rps-10 null. Heterozygous rps-10(cc2557)/tmC20 animals are delayed in development. Reference: Cenik ES, et al. Dev Cell. 2019 Mar 25;48(6):811-826.e6. doi: 10.1016/j.devcel.2019.01.019. PMID: 30799226. |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001067 | dpy-5 | F27C1.8 | Caenorhabditis elegans |
| WBGene00004479 | rps-10 | D1007.6 | Caenorhabditis elegans |
| WBGene00006753 | unc-14 | K10D3.2 | Caenorhabditis elegans |
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