2 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000672 | col-97 | ZK1010.7 | Caenorhabditis elegans |
| WBGene00006843 | unc-119 | M142.1 | Caenorhabditis elegans |
| WormBase ID | WBStrain00062666 | CGC Received | 2025-03-12 |
| Genotype | unc-119(kst33) col-97(wrd346[col-97::internal mNG::3xFLAG + unc-119(+)]) III. | Laboratory | CGC |
| Made By | WBPerson6656 | Mutagen | CRISPR_Cas9 |
| Name | JDW834 | Outcrossed | x0 |
| Remark | Superficially wild type. Modular linker::mNeonGreen::3xFLAG::linker tag inserted in the endogenous col-97 locus by CRISPR to produce a translational fusion inserted internally near the C-terminus (after amino acid 288). Allele obtained using plasmid-based unc-119 selection. Injection of a Cre recombinase plasmid failed to excise the loxP-flanked unc-119(+) cassette for unknown reasons. The insert was verified to be correct through Sanger sequencing. Knock-in is linked to the temperature-sensitive unc-119(kst33) allele. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. | Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000672 | col-97 | ZK1010.7 | Caenorhabditis elegans |
| WBGene00006843 | unc-119 | M142.1 | Caenorhabditis elegans |
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