1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00008852 | ubql-1 | F15C11.2 | Caenorhabditis elegans |
| WormBase ID | WBStrain00054751 | CGC Received | 2023-01-10 |
| Genotype | ubql-1(utx69[mScarlet-I-C1::3xMyc::ubql-1]) I. | Laboratory | CGC |
| Mutagen | CRISPR_Cas9 | Name | GLW89 |
| Outcrossed | x0 | Remark | N-terminal tag of UBQL-1 via CRISPR/Cas9 knock-in of mScarlet at ubql-1 locus. Insertion verified by PCR and fluorescence. Genotyping primers: fwd 5 AGGGCGAGAGATTATCGGGA 3; rev 5 CGGATCGTTGAGAATGTGTCC 3. Left flank: 5' gtcggttttttaatatttctcaaatttaag 3'; Right flank: 5' ATGGCTACAGAGAGTGCACTCATCAAAGTTCACGTGAAATCACCCT 3 (7 silent mutations); sgRNA: TCAACGTCATACTTGTTCGA; Cas9/sgRNA plasmid: pGLOW134; mScarlet^SEC^3xMyc plasmid: pGLOW145; SEC insertion allele strain: GLW88. |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00008852 | ubql-1 | F15C11.2 | Caenorhabditis elegans |
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