WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Strain :

WormBase ID	WBStrain00054809	CGC Received	2023-09-11
Genotype	lag-2(q1049[lag-2::3xV5]) V.	Laboratory	CGC
Made By	WBPerson123	Mutagen	CRISPR_Cas9
Name	JK6099	Outcrossed	x2
Remark	3xV5 tag inserted at C-terminus of endogenous lag-2 locus. Hermaphrodite DTC stain well. Animals are fertile and look wild-type. tracr + crRNA targeting the C-terminus of LAG-2 was injected with Cas-9 protein and repair template to insert a 3xV5 tag, following Fire and Seydoux lab RNP co-CRISPR strategy (with unc-58 crRNA and repair oligo to mark edited offspring).	Species	Caenorhabditis elegans