2 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001067 | dpy-5 | F27C1.8 | Caenorhabditis elegans |
| WBGene00004268 | rab-5 | F26H9.6 | Caenorhabditis elegans |
| WormBase ID | WBStrain00052039 | CGC Received | 2022-03-30 |
| Genotype | rab-5(udn15)/tmC18 [dpy-5(tmIs1200)] I. | Laboratory | CGC |
| Mutagen | CRISPR_Cas9 | Name | UDN100037 |
| Outcrossed | x2 | Remark | rab-5 [Q78Q]/tmC18 I. Control edit mutation maintained over tmC18. Balancer marked with myo-2p::Venus. Heterozygotes are WT with pharyngeal Venus fluorescence, and segregate Venus+ heterozygotes, non-Venus rab-5 [Q78Q] homozygotes (viable and fertile), and Dpy Venus+ tmC18 homozygotes. Pick fertile wild-type Venus+ to maintain. NOTE: udn15 is essentially wild-type. Pick Venus+ to prevent non-Venus rab-5 [Q78Q] homozygotes from taking over the population and losing the balancer! Silent KpnI site added in Q78Q allele for ease of genotyping. Reference: Huang et al. 2022. PMID: 35121658 |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001067 | dpy-5 | F27C1.8 | Caenorhabditis elegans |
| WBGene00004268 | rab-5 | F26H9.6 | Caenorhabditis elegans |
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