2 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000254 | bli-4 | K04F10.4 | Caenorhabditis elegans |
| WBGene00006910 | vha-1 | R10E11.8 | Caenorhabditis elegans |
| WormBase ID | WBStrain00051769 | CGC Received | 2021-06-09 |
| Genotype | vha-1(luc161) III/hT2[bli-4(e937) let-?(q782) qIs48] (I;III). | Laboratory | CGC |
| Mutagen | CRISPR_Cas9 | Name | MLC2230 |
| Outcrossed | x0 | Remark | Heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP, arrested hT2 aneuploids, and non-GFP luc161 homozygotes (embryonic lethal). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick wild-type GFP and check for correct segregation of progeny to maintain. vha-1(luc161) is a 454 bp deletion removing most of the coding sequence. Reference: Gutierrez-Prez, P. et al. A deeply conserved miR-1 dependent regulon supports muscle cell physiology. bioRxiv, 2020, doi.org/10.1101/2020.08.31.275644. |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000254 | bli-4 | K04F10.4 | Caenorhabditis elegans |
| WBGene00006910 | vha-1 | R10E11.8 | Caenorhabditis elegans |