2 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001072 | dpy-10 | T14B4.7 | Caenorhabditis elegans |
| WBGene00017546 | rpa-1 | F18A1.5 | Caenorhabditis elegans |
| WormBase ID | WBStrain00051042 | CGC Received | 2021-03-01 |
| Genotype | rpa-1(iow117)/mIn1[mIs14 dpy-10(e128)] II. | Laboratory | CGC |
| Mutagen | CRISPR_Cas9 | Name | SSM596 |
| Outcrossed | x1 | Remark | Crispr/Cas9-engineered indel in the 5 region of rpa-1. Larval-lethal mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are wild-type with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP iow117 homozygotes (larval lethal). Pick wild-type dim GFP and check for correct segregation of progeny to maintain. iow117 was generated in mre-11::GFP background and outcrossed to N2. Reference: Hefel et al., Nucleic Acids Res. 2021 Jan 21;gkaa1293. doi: 10.1093/nar/gkaa1293. |
| Species | Caenorhabditis elegans |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00001072 | dpy-10 | T14B4.7 | Caenorhabditis elegans |
| WBGene00017546 | rpa-1 | F18A1.5 | Caenorhabditis elegans |
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