WormMine

WSWS298

An integrated data resource for C. elegans and related nematodes

Strain :

WormBase ID  WBStrain00022449 CGC Received  2014-11-11
Genotype  fbf-2(ax2057[fbf-2::GFP]) II. Laboratory  CGC
Made By  WBPerson13788 Mutagen  CRISPR_Cas9
Name  JH3201 Outcrossed  x0
Remark  Maintain at 20-25C. ax2057 was produced by mutation of the sgRNA site and insertion of GFP cDNA at the C-terminus of fbf-2. Insertion of GFP cDNA (from pCM1.53, no ATG/no STOP) at the C-terminus of fbf-2: ATCATCGCCGTGACTACCA(GFP) between II:6089145...6089165. sgRNA site was mutated to avoid Cas9 re-cutting. Reference: Paix A, et al. Genetics. 2014 Sep 23. Species  Caenorhabditis elegans
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1 Alleles

Public Name
ax2057

1 Data Sets

Name URL
WormBaseAcedbConverter  

1 Genes

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00001402 fbf-2 F21H12.5 Caenorhabditis elegans